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  • 產品名稱:熒光素酶(螢火蟲)

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  • 產品廠商:通蔚生物
  • 產品價格:9380
  • 產品庫存:180
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詳情介紹:

中文名稱 :熒光素酶(螢火蟲)

其他中文名稱 : 熒光素酶;重組型酵素

英文名稱 : Luciferase firefly

其他英文名稱 : Luciferase from Photinus pyralis (firefly)

產品規(guī)格 : BR,98%

C A S :61970-00-1

級 別 :BR

product line :BioUltra

Assay :≥98% (SDS-PAGE)

Mol wt :mol wt 120 kDa (two non-identical subunits, each containing four free thiol groups, one of which is necessary for activity)

Protein :10-35% E1%/280

Measuring range :≤1 femtomole ATP sensitivity, (using 0.2 μg of luciferase and suitably sensitive liquid scintillation counters or luminometers)

Foreign activity :ATPase ≤5 nanomolar units/mg protein;Nucleoside diphosphokinase ≤20 nanomolar units/mg protein

活力定義 :One light unit produces a biometer peak height equivalent to 0.02 μCi of 14C in PPO/POPOP cocktail. Light units measured in 50 μl assay mixture containing 5 pmol ATP and 7.5 nmol luciferin in Tris-glycine buffer, pH 7.6, at 25 °C

產品描述 :Two contaminant, ATP-consuming activities are assayed for in this product, ATPase and nucleoside diphosphokinase. These impurities are found to be less than 5 nanomolar units/mg protein and less than 20 nanomolar units/mg protein, respectively.
Note: Prior to 1991, a unit of firefly luciferase activity was defined as that amount which will produce 1.0 nanomole of pyrophosphate per minute at pH 7.7, 25 °C, using a system containing 0.6 mM ATP and 0.1 mM D-luciferin. The former nanomolar unit is equivalent to approximately 1.3 x 106 light units

性 狀 :粉末。EC:1.13.12.7

用 途 :生化研究。

保 存:-20℃。保質期1年

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